In response to the incident, Pope Leo XIV expressed his sorrow and reiterated calls for a ceasefire in Gaza. Netanyahu stated that an investigation is currently underway and reiterated Israel's commitment to safeguarding civilians and religious sites. The Israel Defense Forces (IDF) subsequently reported that preliminary inquiries indicated the church was inadvertently struck by shell fragments during military operations. They asserted that their target selection prioritizes military objectives while trying to limit collateral damage.

Reactions extended beyond Israel, as U.S. officials, including President Donald Trump, expressed disappointment regarding the attack. During a phone conversation with Netanyahu, the incident was characterized as a "mistake." The Latin Patriarchate, representing the affected community, called for an immediate end to the conflict, emphasizing that many had sought refuge in the church to escape the devastating impacts of war, only to face further tragedy.

This event has reignited discussions surrounding civilian safety and the ongoing conflict between Israel and Palestine, making clear the urgent need for dialogue and resolution.

Overall, the strike on the Holy Family Church has underscored the deep humanitarian crises ongoing in the region and the farewell to innocent lives amidst the turmoil of war.